What did the researchers
consider when inoculating Morus alba?
A crucial part of this research
was stage 0 of in vitro culture which
dealt which plant selection and disinfection. For that reason, only healthy,
young growing shoot containing tips and nodal segments were randomly collected
from 3-year-old field-grown ‘Shidareguwa’ trees. In order to create optimal growth
conditions the nutrient composition of the media and the chemicals used to
sterilize the plantlets were tested in pre experiments. (Aroonpong P., Chang
J., 2015)
Which measures are
available for shoot induction and rooting in vitro?
The induction of shoots and
roots can be controlled by the selection of Medias with different concentrations
of phytohormones and nutrients. For an optimal growth shoots and roots dependent
on an amount of 3% of sucrose (Sajeevan et al., 2011) and 0,8% agar in the
media and adjusted to pH 5.8. (Aroonpong P., Chang J., 2015)
Which conclusions do you
make when reading Figure 2 of the publication?
In Figure 2 clearly shows a
direct correlation between the Survival rate and endophytic contamination of
the explants. The reason therefore leads to the meristem of the shoot tips
which aren’t contaminated with endophytic bacteria yet. Node 3 shows a higher
contamination rate as its closer to the media which is the main food source for
fungi and bacteria. In my opinion the increased browning on nodes 1 and 2 could
indicate that they are weaker compared to the shoot tip and node 3 and
therefore more often targeted by pathogens. (Aroonpong P., Chang J., 2015)
Which factors are crucial for the last step of in
vitro culture: acclimation?
An important factor of
stage 4 of in vitro culture is the gently acclimation of the plantlets to their
new surroundings. Therefore, the young plants are covered with a plastic tube
and in order to adapt to different conditions. Furthermore, an optimal temperature
in the green-house and a well grown root system is crucial to avoid a shock in
their metabolism. (Aroonpong P., Chang J., 2015)
Aroonpong, P., Chang J., 2015, Micropropagation of a difficult-to-root
weeping mulberry (Morus alba var.
Shidareguwa): A popular variety for ornamental purposes. Scientia Horticulturae
194 (2015), 320–326.
Sajeevan,
R.S., Singh, S.J., Nataraja, K.N., Shivanna, M.B., 2011. An efficient in vitroprotocol
for multiple in mulberry, Morus alba L.
variety V1. Inter. Res. J. PlantSci. 2, 254–261.
