What did the researchers
consider when inoculating Morus alba?
A crucial part of this research
was stage 0 of in vitro culture which
dealt which plant selection and disinfection. For that reason, only healthy,
young growing shoot containing tips and nodal segments were randomly collected
from 3-year-old field-grown ‘Shidareguwa’ trees. In order to create optimal growth
conditions the nutrient composition of the media and the chemicals used to
sterilize the plantlets were tested in pre experiments. (Aroonpong P., Chang
J., 2015)
Which measures are
available for shoot induction and rooting in vitro?
The induction of shoots and
roots can be controlled by the selection of Medias with different concentrations
of phytohormones and nutrients. For an optimal growth shoots and roots dependent
on an amount of 3% of sucrose (Sajeevan et al., 2011) and 0,8% agar in the
media and adjusted to pH 5.8. (Aroonpong P., Chang J., 2015)
Which conclusions do you
make when reading Figure 2 of the publication?
In Figure 2 clearly shows a
direct correlation between the Survival rate and endophytic contamination of
the explants. The reason therefore leads to the meristem of the shoot tips
which aren’t contaminated with endophytic bacteria yet. Node 3 shows a higher
contamination rate as its closer to the media which is the main food source for
fungi and bacteria. In my opinion the increased browning on nodes 1 and 2 could
indicate that they are weaker compared to the shoot tip and node 3 and
therefore more often targeted by pathogens. (Aroonpong P., Chang J., 2015)
Which factors are crucial for the last step of in
vitro culture: acclimation?
An important factor of
stage 4 of in vitro culture is the gently acclimation of the plantlets to their
new surroundings. Therefore, the young plants are covered with a plastic tube
and in order to adapt to different conditions. Furthermore, an optimal temperature
in the green-house and a well grown root system is crucial to avoid a shock in
their metabolism. (Aroonpong P., Chang J., 2015)
Aroonpong, P., Chang J., 2015, Micropropagation of a difficult-to-root
weeping mulberry (Morus alba var.
Shidareguwa): A popular variety for ornamental purposes. Scientia Horticulturae
194 (2015), 320–326.
Sajeevan,
R.S., Singh, S.J., Nataraja, K.N., Shivanna, M.B., 2011. An efficient in vitroprotocol
for multiple in mulberry, Morus alba L.
variety V1. Inter. Res. J. PlantSci. 2, 254–261.
Hi ho
AntwortenLöschenIt is refreshing to read a review of the same paper I have been dealing with. You absolutely got the significant details and present it in a lively way. The questions are answered brief and accurate, you express yourself scientifically and the content is holistically reproduced. I assume even without reading the paper you could learn something from it. Well done.
Regarding the layout i suggest to highlight the questions and perhaps even make the font bigger. Anyway, I enjoyed reading your paper. Keep it up! (and have fun)
Hi
AntwortenLöschenFirst of all I think you answered the questions in a clear and comprehensible way. It looks like you got all the important points. Furthermore, you cited the original paper by every trick in the book. Well done!
Maybe next time you could write a short introduction just to awaken the interest of the readers and to make clear what the blog entry is about. But all in all I found your blog entry was very informative and pleasant to read. Thanks for that.
Cheers
Hi Stefan, you carefully studied the paper and I find a couple of well reasoned conclusions in your blog. For two points I'd like to add my remarks: (1) reporting media composition and applied PGR you remain quite vague and (2) when commenting figure 2 I only confirm the first point of your conclusion(young or meristematic tissues are less contaminated by endophytes). Browning and higher contamination rates at lower nodes seems to be a natural symptom. These older parts on the plant are not weaker but simply more contaminated by basically not harmful endophytes which are disturbing the in vitro-life of the explant. Cheers Hansruedi
AntwortenLöschen